Structure of some peptides isolated from chymotryptic digest of dolphin myoglobin
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چکیده
منابع مشابه
The immunological activity of some of the chymotryptic peptides of sperm-whale myoglobin.
1. Sperm-whale apomyoglobin was digested with chymotrypsin in a dialysis sac. The ultrafiltrate contained incompletely hydrolysed fragments which partially inhibited the precipitation of metmyoglobin and apomyoglobin by some antisera produced against metmyoglobin. The inhibitory activity was stable to heating at 100 degrees and depended on the peptide structure. 2. The fragments were fractionat...
متن کاملChymotryptic digest of papain. III. Amino acid sequence of six peptides.
Dinitrophenylation-The amino-terminal residue was determined on. a sample of the peptide before desalting. Approximately 1 pmole of the peptide was submitted to dinitrophenylation for 6 hours with the reaction mixture maintained at pH 8.5 with the aid of a pH stat. The solution was evaporated to dryness and triturated with dry ethyl acetate. Estraction was continued until all the yellow color w...
متن کاملextraction and acetylation of purified trypsin from bovin pancreas and study of some its physico-chemical properties
آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...
15 صفحه اولPrimary Structure of the Calcium Ion-Transporting Adenosine Triphosphatase of Rabbit Skeletal Sarcoplasmic Reticulum SOLUBLE PEPTIDES FROM THE a-CHYMOTRYPTIC DIGEST OF THE CARBOXYMETHYLATED PROTEIN
The isolation of the soluble peptides from the chymotryptic digest of the calciumtransporting ATPase of rabbit skeletal sarcoplasmic reticulum is described. These peptides were partially sequenced and the information obtained was used to align tryptic peptides of the protein and to confirm sequences within the tryptic peptides. Details of the isolation of some peptides and the amino acid analys...
متن کاملTryptic digest of papain. I. Isolation and sequence of some peptides from the oxidized protein.
Investigations are in progress in this laboratory to elucidate the amino acid sequence of the proteolytic enzyme, papain (2). Results have already been presented on the isolation and sequence of some peptides obtained after chymotryptic digestion of oxidized papain (3-5). In the present paper, we report the isolation, composition, and sequence of some peptides produced by tryptic digestion of o...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1968
ISSN: 0014-5793
DOI: 10.1016/0014-5793(68)80032-8